Coding

Part:BBa_K4960038:Design

Designed by: ShuChen Xi   Group: iGEM23_NUDT-CHINA   (2023-10-11)


pPVC promoter->pvc1-pvc12->pvc13_NTD-1*EAAAK-CKGGRAKDC-1*EAAAK -pVc13_CTD->pvc14-pvc16


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7406
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2407
    Illegal BglII site found at 11264
    Illegal BglII site found at 14099
    Illegal BglII site found at 16960
    Illegal XhoI site found at 2691
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 11792
    Illegal NgoMIV site found at 17491
    Illegal NgoMIV site found at 17630
    Illegal AgeI site found at 6704
    Illegal AgeI site found at 9641
    Illegal AgeI site found at 10327
    Illegal AgeI site found at 10468
    Illegal AgeI site found at 11576
    Illegal AgeI site found at 17929
    Illegal AgeI site found at 19617
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use the CKGGRAKDC to target fat cells. The different linker(1*EAAAK or 3*EAAAK) on both sides of the CKGGRAKDC can affect the structure and function of the tail protein. By altering the linker on both sides of the CKGGRAKDC, we explored the most suitable structure of the tail protein for recognition and bond. At the same time, because of the existence of linker, we can use Golden Gate to replace the sequences in the middle of linker more efficiently than enzyme cutting and enzyme linking.


Source

Synthetic

References